FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l01n02_ogla_68.3520000009baea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l01n02_ogla_68.3520000009baea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22267378
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG145300.017.4251025
GTATAGG98750.017.1303061
GTATTGG205450.015.8429731
TAGACTG71650.015.3810345
GTATAAG100950.014.6858991
CCTAGAC76900.014.6410033
TATACCG73750.014.4277865
TAGACAG81150.014.3999295
CCTATAC97650.014.3028943
CCTACAC101850.014.2261583
GTCTAGG81100.013.8860821
CTATACT145750.013.8850344
GTATATA307200.013.6736131
GTATAGA122700.013.6704381
TACACTG104400.013.5590565
GTGTAGG100250.013.5560731
ACAGTGC117800.013.546588
TACACCG77550.013.2920415
TGCGTTC141050.013.2666659
GTATTAG116050.013.2254241
Produced by FastQC (version 0.11.2)