FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l01n02_ogla_65.3520000009bc55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l01n02_ogla_65.3520000009bc55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31179430
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG205850.016.799445
GTATAGG142750.016.0777021
GTATTGG252700.015.0995241
GTATAAG146050.014.5756991
CTATACT202950.014.3486654
GTGTAGG134400.014.2128031
TATACAG182100.014.1385115
TACACCG107250.014.1288795
TAGACTG98300.013.7722745
GTATATA449800.013.6805411
GTATTAG164900.013.6587411
CCTATAC136850.013.3647733
ACTGTGC141650.013.3109028
TATACCG104250.013.25962455
TACACTG153700.013.2276955
TACACAG144550.013.1120055
TCTATAC166800.012.9302073
CCTAGAC103550.012.5703133
GTATAGA173400.012.4685311
TAGACAG107800.012.3823165