FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l01n02_ogla_64.3520000009bcce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l01n02_ogla_64.3520000009bcce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31695880
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG208700.016.615545
GTATTGG297850.016.2402631
TATACCG102500.015.2934035
GTATAGG146350.014.9675631
TACACTG149100.014.7508615
GTATAAG145600.014.6530441
CCTATAC145900.014.2614273
CTATACT206450.014.2204384
TACACCG107450.014.1467795
CTACACT148500.013.9795874
TATTGGG322300.013.9438242
TAGACTG104950.013.8953655
GTGTAGG144750.013.7542941
CCTACAC134650.013.4066863
TAGACAG118150.013.3882725
TATACAG188600.013.2482375
GTATATA448250.013.239911
ACTGTGC142850.013.0328398
TGCGTTC192150.013.0259349
GTCTAGG108250.012.9929171
Produced by FastQC (version 0.11.2)