FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l01n02_ogla_62.3520000009bde7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l01n02_ogla_62.3520000009bde7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21162157
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG214600.015.6546261
TATACTG131900.015.4141865
GTATAGG94950.015.2636321
TATTGGG240450.013.7909222
GTATATA288950.013.7643521
TGCGTTC144100.013.7108649
TACACTG95650.013.5581155
TAGACTG68400.013.542545
TATACCG67800.013.4521955
GTATAAG93450.013.1696271
TATACAG122350.012.8512595
CTATACT135000.012.8443014
GTCTAGG70750.012.7608671
ACTGTGC97000.012.632188
GTATAGA115800.012.5974561
GTGCGTT162800.012.4567218
TACACCG72800.012.3977785
ATTGGGT233200.012.3452123
TTATACT155950.012.3068644
GGTGCGT174050.012.3067127