FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l01n01_ogla_68.3510000009baed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l01n01_ogla_68.3510000009baed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22267378
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG145250.018.3790665
CCTAGAC73500.015.8984563
GTATTGG200650.015.7717111
GTATAAG102100.015.6836771
GTGTAGG103150.015.5240271
GTATAGG98700.015.3573861
TAGACTG76900.015.1336275
TATACCG72000.014.9100535
CCTATAC101550.014.7813593
TACACCG78950.014.3195095
TATACAG127400.014.1310045
TACACTG103750.014.0557355
ACTGTGC101550.013.9372548
CTATACT148300.013.9011864
GTATATA312200.013.621781
TACACAG96600.013.5225575
TATTGGG218750.013.2460692
TAGACAG83200.013.1313025
GTGCGTT147750.012.9865788
ACAGTCC58650.012.9566538