FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l01n01_ogla_67.3510000009bb65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l01n01_ogla_67.3510000009bb65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56379146
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG363100.015.9735555
GTATTGG465600.015.093711
TATACAG311400.014.7356965
TATACCG168250.014.6811625
GTATAGG241050.014.5672661
ACTGTGC235050.014.5079568
TACACTG262550.014.4559585
GTATATA833150.013.7960081
GTATAAG264700.013.5349981
TACACCG167300.013.3732545
GTATTAG298650.013.2373671
TTATACT455450.013.2144174
CTATACT371050.013.2117214
TAGACAG195100.013.196365
GTCTAGG191200.013.1713051
GTATAGA317100.013.0515681
CCTAGAC176650.012.8536933
CCTATAC233650.012.828543
CTACACT254150.012.6721914
TAGACTG175600.012.605895