FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l01n01_ogla_65.3510000009bc58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l01n01_ogla_65.3510000009bc58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31179430
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG204100.016.4079115
TATACCG101750.015.4058875
TATACAG183500.014.5481395
TAGACTG98550.014.3637175
GTATTGG256900.014.2791311
ACTGTGC145900.014.0952548
GTATAAG141100.013.9418981
GTATATA462150.013.8803181
TACACCG106950.013.6353035
GTATAGG139500.013.6249341
TAGACAG110450.013.1602125
CCTATAC140050.013.0583073
GTCTAGG118050.012.96099951
TTATACT248750.012.8134654
GTATATG288100.012.7492561
CCTAGAC106550.012.6611793
TACACTG155500.012.5550545
CCTACAC133650.012.5462813
GTCCTAG114550.012.5273861
GTATTAG157800.012.4965381
Produced by FastQC (version 0.11.2)