FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l01n01_ogla_63.3510000009bd20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l01n01_ogla_63.3510000009bd20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57523606
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG352500.016.8312785
TATACCG169300.016.0211225
GTATAGG236650.015.420981
TATACAG311550.015.2623045
GTATATA739250.014.8869081
GTATTGG438750.014.7725171
CCTATAC232050.014.6775653
TACACTG251150.014.6015155
CTATACT352900.014.4431484
TAGACTG176900.014.2318655
GTATAAG250950.014.20140651
CCTAGAC181550.013.9720923
ACTGTGC242700.013.8158428
TACACCG189200.013.733465
TACACAG235500.013.7161365
GTATAGA305250.013.5120531
TCTATAC287150.013.2011123
GTATTAG274750.013.0058111
GTGTAGG233000.012.9705521
TAGACAG196250.012.8528265