FastQCFastQC Report
Tue 13 Feb 2018
CAEYYACXX_l08n02_smnv0027bronchsup.352000000bdb5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l08n02_smnv0027bronchsup.352000000bdb5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26223531
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC828210.3158270333617544No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC578270.22051568875297534No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG504020.1922014239806226No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG410890.1566875185496568No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG387280.14768415435739757No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC382200.14574696290899955No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG342860.13074516929089372No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG293690.11199483395275792No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC277930.10598496441993262No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG274650.10473417939025832No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC267940.10217540879601605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGC121250.061.4007071
GTTCGAC87300.060.034841
TTCGACC101200.051.2960362
CCCCGTG129550.044.244771
CCGAGGA131950.040.3873672
TGGCAGG130850.039.819351
GCCGAGG201250.039.2034031
GGCAGGT148050.038.8943142
GCCGCGA105500.033.2964551
CGGCGAG128200.031.0701351
CCGCGAG103600.030.1829932
GTTGCGG118050.029.756681
GCCGGAG96900.028.3363691
TTGCGGA115950.028.0257322
GGCGCGA126150.026.9342582
TCCGACC54100.024.9517141
CGGGGGA227400.023.8571531
CCGGAGG108700.022.0451932
CCGAGGG106700.021.8837812
CTTGGGC129900.021.8069211
Produced by FastQC (version 0.11.2)