FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l07n01_smnv0005bronchsup.351000000bdb94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l07n01_smnv0005bronchsup.351000000bdb94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32073416
Sequences flagged as poor quality0
Sequence length101
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC7624372.377161821491044No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG4890781.5248703162768817No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC4726201.4735567923291988No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG4380071.3656387582788188No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG4241071.322300686649654No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC3698571.1531574934207196No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA3625671.1304283896670064No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC3320821.0353808275364247No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG3120020.9727744621901202No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC3093420.9644809894898628No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC2997470.9345652486782201No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG2748240.8568591508930636No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG2723330.8490925943154917No Hit
GCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCC2636490.8220172120113429No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC2487380.7755269971867044No Hit
TTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGT2224790.6936554559701406No Hit
GCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAA2224440.6935463313293476No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC2186130.681601859932849No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC2120950.6612797339703386No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC1866790.5820365376734427No Hit
CCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCC1716860.5352906594046609No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG1625870.5069213706453968No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC1601050.49918287468974304No Hit
GCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCC1458270.4546662569400154No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG1428220.4452971270662283No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1423490.44382238549208475No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCG1407400.438805769862493No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC1359350.42382451560507306No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG1340150.4178382495958647No Hit
GGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGT1225410.38206407449708507No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG1205150.37574731671861833No Hit
GAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCG1172470.3655581931154449No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG1168870.3644357682387183No Hit
TCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC1161810.36223456834158235No Hit
CGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGC1031860.321718148138633No Hit
CCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCG1018510.31755582255410525No Hit
CTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCG1003090.31274810266545977No Hit
CGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC994640.31011352205203213No Hit
CGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCG978140.30496907470036866No Hit
TGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGC949480.29603332554287326No Hit
GCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCG938930.29274399708468846No Hit
CCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGG885030.2759388024025879No Hit
GCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGC837690.26117891527363346No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG833780.2599598371436332No Hit
GCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGT824910.25719430696125417No Hit
GCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCG795600.24805589775657197No Hit
CGAGGAGGCAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG766820.23908273443651903No Hit
CGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCC763450.23803202003802776No Hit
CCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCC751220.23421889330403722No Hit
GCGAGGAGGACGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC748050.23323053584314188No Hit
GCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCC731250.22799255308508454No Hit
CGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCC725040.22605637017273122No Hit
GCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGG715530.2230912977900452No Hit
CGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAAC712410.22211852956354883No Hit
TTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGA705140.2198518548819371No Hit
CGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG704150.21954318804083733No Hit
GTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGG693750.21630062728584942No Hit
CTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCC683010.21295205973694853No Hit
CCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGT673980.2101366440044927No Hit
GCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGC670280.20898304065896817No Hit
CGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTC640770.19978227451669006No Hit
GTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCG636890.19857254992732923No Hit
GGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCC634830.19793027347009126No Hit
CGGGCGTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGC619910.19327844592543558No Hit
GAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGC604550.18848943311806887No Hit
CCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGT603640.18820570905200745No Hit
CCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGG603000.1880061668517005No Hit
TTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGG599750.18699286661576678No Hit
CGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGA597820.18639112216796613No Hit
CCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGA565460.1763017696649462No Hit
CGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGC564550.17601804559888476No Hit
GGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGA554110.1727630134563777No Hit
GGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCG548570.1710357262849707No Hit
GTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCT547650.17074888437202945No Hit
CGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGC546630.17043086399029025No Hit
GCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTT543730.16952668839514942No Hit
CGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCC531110.16559196563284684No Hit
CCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCAC523390.16318498784164429No Hit
CGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGC522240.16282643545046777No Hit
GTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGC504980.15744503173593982No Hit
CTCGACACAAGGGTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGGG502010.1565190312126404No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGC499190.15563979839253791No Hit
GCGGGCGTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCG499070.15560238422998035No Hit
GGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCG495280.15442072026253767No Hit
GCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCC494150.15406840356512072No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT492670.1536069622269109No Hit
CGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCC486980.15183290735230698No Hit
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG485130.1512561056795447No Hit
CCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACG481510.15012744510905854No Hit
GCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCC476050.1484251007126899No Hit
CCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTC470850.14680382033519596No Hit
GTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCC464760.1449050515854002No Hit
GTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGT460860.14368909130227975No Hit
GGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCC456790.14242012762220277No Hit
CTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGC449970.14029375605018188No Hit
GTTTGGGCGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG443200.13818297371255994No Hit
CCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTC438580.1367425284540942No Hit
CGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGC437840.13651180778498928No Hit
GGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCG436800.1361875517094905No Hit
CGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTC435310.1357229925244009No Hit
GACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCG424790.13244301760685545No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGCCCCCGGG424190.1322559467940677No Hit
CCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGC423650.13208758306255872No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG418660.1305317774695405No Hit
GTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGG417340.13012022168140744No Hit
TTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCC415730.12961824833376026No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGCGGGCCG413490.12891985063268596No Hit
CGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGG411220.12821209939097225No Hit
CGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGC410970.12813415321897736No Hit
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC400840.124975774329744No Hit
CGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCC398950.12438650126946253No Hit
CCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGGG398690.12430543725058783No Hit
CGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAAC392350.12232872232879717No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT390120.12163344247460264No Hit
GCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCG385910.12032082893820853No Hit
TGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTC381170.11884296951718519No Hit
CGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTC378540.11802297578779883No Hit
CCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGG377310.11763948062158394No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC375670.11712815373329738No Hit
GGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGG375140.1169629078486682No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT374540.11677583703588043No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCC372700.11620215320999797No Hit
AGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCC371690.11588725067513857No Hit
GGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTC354730.11059938236700449No Hit
GGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGG352920.11003505208176143No Hit
GGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCC351340.10954243227475363No Hit
GCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCC351310.10953307873411426No Hit
GGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCC348660.10870684931096831No Hit
GCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGC346780.10812069409756667No Hit
CGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTT344770.10749400687472764No Hit
CTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGG340410.10613462563513658No Hit
CCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTG338400.10550793841229758No Hit
GGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCT337050.10508702908352513No Hit
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGG335520.10460999851091633No Hit
CCTCGACACAAGGGTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGG333670.10403319683815405No Hit
CGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGG332700.10373076569081385No Hit
CTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGA331730.10342833454347364No Hit
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGG323470.10085299302076212No Hit
CTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGG322500.10055056187342191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC49400.062.9034231
GTCGGTT9300.058.7541241
CTGGGCT12750.055.899061
CTTGGGC263400.055.5234341
AGGCAGG146400.054.0150956
AACCGAC43650.052.227475
GCGTACG61100.051.0115432
GGAGGCA156450.050.8796124
GGCAGGA158400.050.3428157
GAGGCAG158850.050.2002035
CCTGCCT22550.048.6730881
CGTACGG65800.047.133823
CCGACGA48700.046.8116847
ACCGACG50000.045.9745376
CGACGAA62400.045.134868
GCAGGAC179400.044.7675328
ATTGGGC7400.043.66171
GGCTGCC64500.043.0762755
CTGGTCG14900.042.7308961
ATTCGGT7850.042.3693541
Produced by FastQC (version 0.11.2)