FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l07n01_copd0021balruntouched.351000000bda88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l07n01_copd0021balruntouched.351000000bda88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42489268
Sequences flagged as poor quality0
Sequence length101
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC10372992.4413200057953457No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG6618711.557736885464819No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC6368381.4988208316509477No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG6038741.421238887899881No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG5792361.3632524806028665No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA4846631.1406715691124638No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC4802991.1304007402528091No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC4367031.0277960072176344No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC4218550.9928507123257572No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG4192310.9866750352112443No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC4042280.9513649423190816No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG3740800.8804105544957848No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG3628840.8540603711977339No Hit
GCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCC3476680.8182489752471143No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC3449960.8119603284292872No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC3007660.7078634538961698No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC2885600.6791361997575481No Hit
GCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAA2879310.6776558259370342No Hit
TTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGT2860050.6731229165915497No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC2671150.6286646312664177No Hit
CCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCC2293990.5398986868872394No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG2279780.5365543129620401No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC2107790.4960758561432501No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC1945890.4579721166295452No Hit
GCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCC1935810.4555997528599457No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG1927130.45355688405834627No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCG1876260.4415844490425206No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG1861190.43803767106554997No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC1853380.4361995598512076No Hit
GGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGT1635130.3848336478755059No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG1623870.38218356691859223No Hit
GAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCG1594660.37530888976482246No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG1586630.3734190007697944No Hit
TCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC1551550.3651627982859107No Hit
CTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCG1423580.33504460467523234No Hit
CCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCG1411710.33225095805369015No Hit
CGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC1372880.3231121797626638No Hit
CGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGC1359530.319970209889236No Hit
CGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCG1319610.3105748962302669No Hit
TGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGC1272160.29940737035055537No Hit
GCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCG1244200.2928268851324998No Hit
CCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGG1231970.28994851123347193No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG1180290.2777854398433035No Hit
GCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGC1146330.26979283333381976No Hit
CGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCC1050890.2473306906581681No Hit
GCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGT1044450.24581501380536844No Hit
CGAGGAGGCAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG1041310.2450760036628543No Hit
CGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTC1019280.23989116498782706No Hit
GCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCG1017910.23956873062628428No Hit
CTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCC995760.2343556495254284No Hit
GCGAGGAGGACGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC991790.23342129593760005No Hit
CCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCC983550.23148198269737197No Hit
GTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGG961330.2262524268481161No Hit
CGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAAC958480.22558166923468767No Hit
CGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG951730.22399303278183091No Hit
CGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCC948570.22324931556834537No Hit
CCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGT937960.22075221441800316No Hit
TTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGA923300.21730193139594686No Hit
GCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGG916000.21558385049137585No Hit
GCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCC908670.2138587089803477No Hit
GCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGC864710.20351256698515025No Hit
GGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCC843060.19841716265858006No Hit
GTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCG839980.1976922737289802No Hit
CCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGG837740.19716508178018033No Hit
CGGGCGTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGC836100.1967791019605233No Hit
CCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGT834820.19647784941835195No Hit
GAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGC818210.19256862697658148No Hit
CGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGA816170.19208850573749586No Hit
CGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGC792800.1865882933073829No Hit
CCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGA791490.18627998015875444No Hit
TTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGG774100.1821871819490983No Hit
CGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGC761760.17928291915972758No Hit
GCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTT759930.17885222216584198No Hit
GGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCG745720.1755078482406428No Hit
GGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGA742320.17470764617550014No Hit
CCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCAC739680.17408631280727171No Hit
CGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGC735660.1731401915420148No Hit
GCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCC720950.16967814084252994No Hit
GTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCT718890.16919331253247288No Hit
CGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCC718140.16901679737104436No Hit
GTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGC711590.1674752316279019No Hit
GCGGGCGTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCG694450.1634412718053886No Hit
CTCGACACAAGGGTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGGG687630.16183616060413186No Hit
GTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGC685990.16145018078447482No Hit
GGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCG684050.16099359490024634No Hit
GTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGT665720.15667956435493313No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT662280.1558699481478476No Hit
CTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTG658860.15506503901173352No Hit
CGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCC651540.15334225103619106No Hit
CCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTC651440.15331871568133393No Hit
GCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCC645810.15199367520287713No Hit
CTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGC631170.14854809925179224No Hit
CCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACG621380.14624398801127853No Hit
TTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCC613230.14432585659042185No Hit
GTGGTGGTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCC613170.14431173537750758No Hit
GGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCG588060.13840200777288045No Hit
GGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCC587360.13823726028888048No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGCCCCCGGG585810.13787246228859484No Hit
GTTTGGGCGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG580040.13651447231333805No Hit
GACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCG576190.13560836115133826No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG575350.13541066417053832No Hit
CCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTC574350.13517531062196694No Hit
GTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGG574270.13515648233808122No Hit
CGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGC572990.1348552297959099No Hit
CGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTC567170.1334854721432245No Hit
CGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGC566360.13329483576888168No Hit
CGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGG563720.1326735024006533No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGCGGGCCG562310.13234165389716762No Hit
CGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAAC557900.13130374474796788No Hit
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC555350.13070359319911087No Hit
CCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGGG553380.13023994670842529No Hit
CCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGC550530.12956918909499687No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT547990.1289713910816256No Hit
CCTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGG535720.12608360304065488No Hit
CGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCC525340.12364063320648405No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC523680.12324994631585558No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCC518110.12193902705031304No Hit
CGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTC510610.12017387543602776No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT510040.12003972391334207No Hit
AGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCC508730.11973141076471357No Hit
GGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGG497760.11714958233688565No Hit
CGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTT492760.11597281459402876No Hit
TGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTC484830.1141064609538578No Hit
GCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCC483360.11376049123745788No Hit
GGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGG479690.11289674371420097No Hit
GGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCC476780.11221186488785827No Hit
GCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGC476590.11216714771362972No Hit
GCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCG473460.11143049110660132No Hit
GGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCC473250.11138106686140134No Hit
CGTCCTTCCGTTCGTCTTCCTCCCTCCCGGCCTCTCCCGCCGACCGCGGG469260.11044200620260157No Hit
GGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTC468530.11027019811214445No Hit
CTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGG464570.10933819805980184No Hit
GGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCT464170.10924405664037327No Hit
CTCCCTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGG453390.10670694538677392No Hit
CCTCGACACAAGGGTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGG452840.10657750093505963No Hit
CTCCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGA451460.10625271303803116No Hit
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC450620.10605501605723121No Hit
CCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTG440130.10358615733271752No Hit
CCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCG435890.10258825828677491No Hit
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGG435390.10247058151248922No Hit
GCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGAC435060.10239291484146068No Hit
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGG434610.10228700574460355No Hit
CTGCAGGTCGCCCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC432950.10189631885397507No Hit
CCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGA431870.10164213702151799No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGCCC431770.10161860166666087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGGC7100.077.6307751
GTCGGTT10850.072.696361
ATCGGTT9600.070.283181
GGCGTAC64750.066.925151
CACTACG2800.066.1791842
CTGGGCT14200.064.580781
ACTACGC2950.062.7898333
CACTAGC1300.062.1081629
ATGCCCT4700.061.6689151
CTTCGAC4500.060.186161
ATGGGCT3700.059.073191
ATGCAGG7800.057.871311
ATTCGGT6000.057.8103941
CTTGGGC355100.057.7249761
AGGCAGG200150.056.404896
CTGGTCG17100.054.7399521
GGAGGCA213350.053.405044
GAGGCAG213900.053.3341455
GCGTACG82350.052.965612
GGCAGGA214950.052.8305637
Produced by FastQC (version 0.11.2)