FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l06n02_smnv0023balruntouched.352000000bd9f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l06n02_smnv0023balruntouched.352000000bd9f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21322712
Sequences flagged as poor quality0
Sequence length101
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC958830.4496754446620111No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC952310.4466176722735832No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG492100.23078677796708036No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG489730.22967528708355672No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG485360.22762582920971777No Hit
CCCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGG477780.2240709343164228No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC458100.2148413391317202No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC424930.19928515659734092No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA376900.1767598793249189No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG373270.17505746923749663No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG356610.16724420420817016No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC326940.15332946390684263No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC275780.12933626829457717No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC266890.12516700502262565No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC263220.12344583559539707No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCG255450.1198018338380221No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG239060.1121151943523882No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG227930.10689540805128353No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC223850.10498195539104033No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG222560.10437696668228694No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG222540.1043675870123838No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC221140.10371101011916307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC67250.0105.8880541
TTGCGGA47350.078.5797352
GTTGCGG60950.066.2620851
TTCGACC123800.058.7133372
AAGGTCG28300.045.7924772
GGGACGC188100.043.6462441
GTTGCGC15600.043.5790941
TTCGACA21750.041.8404052
CCCCGTG194200.038.922921
CCGAGGA133050.038.397842
TGGCAGG154300.036.852931
CTTTCGG99000.036.4882621
GGCAGGT189900.035.6378062
TTGCGAT8150.033.215312
CGGCGAG187400.033.079851
CGGTACG9850.032.169721
GGTACGT8650.031.295352
CCCGGTA23950.031.2722151
CGGCTAA21200.031.2523561
GTTTTGG82300.029.8215751
Produced by FastQC (version 0.11.2)