FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l06n02_copd0036bronchsup.352000000bda6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l06n02_copd0036bronchsup.352000000bda6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31802540
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC887190.2789682836653927No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC768680.24170396452610388No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG547730.1722283817581866No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG443130.13933792709638917No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG434810.1367217838575158No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC389870.12259083708408196No Hit
CCCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGG388750.12223866395577208No Hit
GCCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCAC373660.11749375993238276No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG333320.10480923850736451No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG319250.10038506358297167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGC124050.064.763581
GTTCGAC105600.056.2535321
TTCGACC121800.049.133872
GCCGAGG213450.044.4744071
CCGAGGA140650.042.9178352
GCCGCGA109200.040.6144141
GGCAGGT170050.040.2443542
CCCCGTG154200.040.13211
TGGCAGG164050.038.3904231
CCGCGAG110900.034.6756972
CGGCGAG140550.034.3045651
GGCGCGA110200.032.698612
GTTGCGG121200.032.120161
GCCGGAG117700.029.2042681
CCGAGGG114300.027.2886412
TCCGACC63700.026.0753141
GGGTCGA102700.024.8215161
CCGGAGG127750.024.1448062
CGGGGGA213850.023.7058811
CTTTCGG96400.023.4523051
Produced by FastQC (version 0.11.2)