FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l05n01_copd0041bronchsup.351000000bdbbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l05n01_copd0041bronchsup.351000000bdbbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33251642
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC3042730.9150615780116963No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC2569310.7726866540906461No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG2319360.697517433875897No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG2152990.6474838144835073No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC2062810.6203633492746012No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG1940100.5834599085362461No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA1579430.47499308455203504No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG1498180.4505582010055323No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC1400800.42127242919312075No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC1322280.39765855773378045No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC1247420.3751453837978888No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG1232330.3706072620413753No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG1205540.3625505170541653No Hit
GCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCC1116890.3358901794985042No Hit
GCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAA1042650.3135634625201366No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC977010.293823083984845No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC931040.2799982027955191No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC904240.2719384504380265No Hit
TTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGT845490.2542701500274783No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC796000.23938667449866086No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC771080.2318923077543058No Hit
GCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCC757800.22789852001895125No Hit
CCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCC730990.21983576029117596No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG718610.21611263588125965No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG709910.2134962237353572No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG698160.20996256365324756No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG694230.20878066713216747No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC658390.19800225203916244No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC648820.19512419867866976No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCG634720.1908838065801382No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG573110.17235539826875318No Hit
GGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGT562760.16924277002621405No Hit
CTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCG538510.1619498970907963No Hit
GAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCG530550.1595560303458097No Hit
TCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC522800.15722531837675865No Hit
CGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCG520440.15651557899005408No Hit
GCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCG515540.1550419675515573No Hit
TGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGC491580.1478363083543363No Hit
CGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC484790.14579430393241935No Hit
CCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCG436470.1312626907266715No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG406790.12233681572777669No Hit
CGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGC387850.11664085641244423No Hit
TTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGA382990.11517927445507804No Hit
GCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGT377000.1133778596557728No Hit
CCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGG376760.11330568276898927No Hit
GCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGC348650.10485196490446999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC54650.032.875241
CTTGGGC174150.030.4584671
AGGTCGA790600.030.3256684
TGCAGGT749800.030.287821
GCAGGTC830850.028.8521352
CGTACGG58300.028.5947573
GCGTACG60500.027.9653432
CAGGTCG893850.026.8705253
GGTCGAC932700.025.623595
GACAGCG58850.024.1309579
TCGACCC1195050.021.7851962
GTCGACC1195950.021.7351281
ACCCGTG1336900.019.9050395
GACCCGT1352800.019.6397844
CGGGGTG51800.019.2688961
TGGGCTG272450.019.2629743
CGACCCG1395400.018.9346923
TTGGGCT281050.018.9222162
GGGCTGC288600.018.2837644
GTACGGA101800.017.1691154
Produced by FastQC (version 0.11.2)