FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l04n02_copd0035bronchsup.352000000bdbae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l04n02_copd0035bronchsup.352000000bdbae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53649276
Sequences flagged as poor quality0
Sequence length101
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC3917080.7301272807483925No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC2821370.5258915330003708No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG2282430.4254353777299809No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG2030110.378403988154472No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG1833020.34166723890178874No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC1807300.33687313879128583No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG1596980.29767037303541616No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG1528960.2849917303637052No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCG1323390.2466743446826757No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1243710.23182232692198868No Hit
TTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGT1237420.23064989730709506No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC1133980.21136911521415497No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA1120490.20885463580160896No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC1027320.19148813862837588No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG999180.18624296066921758No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG963000.17949916043601408No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG952350.17751404511031985No Hit
TTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGG918590.17122132272577173No Hit
CGGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC900690.16788483781216357No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC856620.15967037467569925No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG839470.15647368661601324No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG837320.1560729356347698No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC832830.1552360184692893No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC829070.15453517024162639No Hit
GGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCC818960.15265070865075608No Hit
GGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGT812830.1515081023646992No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC800910.1492862643663635No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC783850.14610635192914811No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC731730.1363914025605863No Hit
CCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTC731370.13632430007070365No Hit
CCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCG681160.12696536669013017No Hit
GAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCG633300.11804446345184602No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG622870.11610035520330227No Hit
TTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGA573930.10697814449537027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC292450.094.501821
TTCGACC309300.089.752222
TGGCAGG307650.087.078531
GTTGCGG240950.079.626531
GGCAGGT363500.078.20672
GGGACGC475350.074.691291
TTGCGGA241250.072.432672
CCGAGGA375500.062.870332
CTTTCGG156300.060.5318831
GCCTGCA163400.053.368451
GCCGAGG759000.053.3285451
GCCGCGA401900.049.748891
GCCGGAG276300.047.7279241
TCCGACC122850.044.901821
CGGCGAG455250.044.5721971
TTTTGGG297600.043.991122
CCGCGAG409500.043.13842
CTTGCAG199150.042.914611
GGGCGTA48050.041.575951
TTGCAGG204350.041.5752072
Produced by FastQC (version 0.11.2)