FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l04n02_copd0021bronchsup.352000000bdabb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l04n02_copd0021bronchsup.352000000bdabb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42851733
Sequences flagged as poor quality0
Sequence length101
%GC78

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC3999620.9333624850131499No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC2930810.6839420006654107No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG2358510.550388475537267No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG2105050.4912403426017799No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG1924430.4490903553422215No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC1811570.42275303078174226No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG1780800.4155724577113369No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG1523960.3556355585432216No Hit
GGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCG1382630.3226543953309893No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1275090.29755856081713195No Hit
TTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGT1256160.2931410031888325No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA1246950.290991731886316No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG1187830.27719532369904387No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC1144270.2670300405353501No Hit
GGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGG1136320.26517480634913876No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC1043410.2434930694634917No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG1014140.23666254057916397No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC971360.22667928039223056No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG958840.2237575782524361No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC957330.22340520043845133No Hit
GCCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC956920.2233095216942568No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC946830.2209548911359081No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC928340.21664001313552475No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG903350.21080827699547178No Hit
TTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGG885700.20668942373929192No Hit
GGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGA882590.2059636654601577No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG877680.20481785415773032No Hit
GGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGT868890.20276659522731555No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC822480.19193622811007433No Hit
CGGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCC791140.1846226382489595No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC791120.18461797099314514No Hit
CCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTC749570.17492174703879537No Hit
CCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCG704270.1643504126192516No Hit
CCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGC696600.16256052001444143No Hit
GAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCG694720.1621217979678908No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG662840.15468219219978804No Hit
CCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCC600370.14010401866360922No Hit
TTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGA599460.1398916585240555No Hit
GGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC582940.13603650522138744No Hit
CTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCG572150.13351852070953582No Hit
TCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC559830.13064349112788506No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG538930.1257662088018704No Hit
CGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCG532410.12424468340638639No Hit
GCCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG531650.1240673276854404No Hit
CCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGG519580.12125063880146923No Hit
TGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGC473650.11053228582377286No Hit
GCCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGG464260.10834100921892705No Hit
CGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGC457910.10685915549786516No Hit
CCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCG456370.10649977680015882No Hit
CGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC453160.10575068224195273No Hit
GGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCC443400.10347306140454109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC256050.0106.9090961
TTCGACC263250.0104.858872
GTTGCGG190050.0100.6717151
TTGCGGA174100.0100.220272
TGGCAGG261750.0100.033991
GGCAGGT320500.086.601172
GCCTGCA111650.082.010781
CTTGCAG108600.079.351341
TTGCAGG113300.077.8432
GGGACGC463000.075.795651
CTTTCGG129850.073.97481
CCGAGGA350350.069.8967362
AAGGTCG93750.068.984982
TTCGACA48500.067.4838942
CTTCGAC52200.066.787751
GTTTTGG232400.064.764411
CAAGGTC100450.064.3281561
TTCCGCT86500.063.0197142
GGGCGTA34950.060.0758821
AAAGGAC98950.059.5726362
Produced by FastQC (version 0.11.2)