FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l03n02_smnv0023bronchsup.352000000bda1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l03n02_smnv0023bronchsup.352000000bda1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38756542
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC737040.19017176506614034No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC569770.14701259983411316No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG515380.13297884006266608No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG426130.10995046978133395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGC141900.045.774721
GTTCGAC117650.041.661381
TTCGACC134150.036.750552
TGGCAGG164400.030.1605171
GCCGAGG282150.028.4688151
GGCAGGT207300.027.6545622
CCGAGGA187150.027.0426062
CCCCGTG182800.024.2596021
GCCGGAG139000.023.0577051
GCCGCGA146000.023.0439591
CGGGGGA277800.021.1070441
TTCCGGG92200.019.8820672
GGCGCGA151600.019.151712
AGGTCGA434550.018.5903265
CGGCGAG241800.018.5049841
GTTGCGG191000.018.3002681
TTGCGGA175000.018.2824692
CCGCGAG153900.017.3858452
GCCCGAG83300.016.8800771
GGTCGAC487400.016.652216
Produced by FastQC (version 0.11.2)