FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l02n02_copd0026bronchsup.352000000bd9c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l02n02_copd0026bronchsup.352000000bd9c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32974363
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC448940.13614819488703997No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC410170.12439057579368554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGC124550.031.7002091
GTTCGAC117650.031.080561
TTCGACC127450.027.2395292
CCCCGTG128850.024.4180851
GCCGAGG194850.023.1125741
CGGGGTG157750.022.1488841
CGGCGAG165450.022.067211
GGCGCGA123800.021.5642852
GGCAGGT179550.021.2096332
CGGGGGA282050.020.7590541
CGGGGTA63850.019.7630861
TGGCAGG143400.019.1246221
CGGGGAA168750.019.1098481
CGGGGAT108050.018.8414821
CGGGCTA59600.018.6316831
CTTTCGG131350.018.4453471
CGGGTGA77750.018.3938641
CGGGCAA93850.017.8677581
CCGAGGA153000.017.5953772
CGGGCAG113050.017.015971
Produced by FastQC (version 0.11.2)