FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l02n01_copd0026bronchsup.351000000bd9cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l02n01_copd0026bronchsup.351000000bd9cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32974363
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC917670.2782980220118278No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC899740.2728604643552932No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG661630.2006498199828758No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG645040.19561863863753792No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA615640.1867026210635214No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG584150.17715277775039961No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC518350.1571978812752198No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC503420.15267012133031954No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC473890.14371467918879888No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG437720.13274555144552755No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC413720.12546716975245284No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT367590.1114775136065555TruSeq Adapter, Index 27 (97% over 39bp)
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG361530.10963972222905413No Hit
CGGGGAGCGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCC358350.10867533665472173No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG337030.10220970758404037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC302450.024.0581385
GTCGACC405000.023.7593021
AGGTCGA305050.023.603974
TCGACCC413300.023.0153122
ACCCGTG453750.021.5524445
CGGGGAT98700.020.5576931
GACCCGT476700.020.4849454
GCAGGTC378000.019.3825442
CGACCCG505400.019.368663
CAGGTCG373550.019.2501433
CCCGTGC568000.017.4257876
CCGTGCG572350.017.193747
CGGGAAC257200.016.5169641
CTGGGGG58500.016.4893631
CCGGGAG188800.016.3848571
GGGGAAT108600.015.6645131
TGCAGGT413150.015.3660551
GTGCGGA645650.015.3080749
CGGGCAT74550.015.2339991
CGGGGTG103700.014.7550541
Produced by FastQC (version 0.11.2)