FastQCFastQC Report
Mon 12 Feb 2018
CAEYYACXX_l01n02_smnv0004bronchsup.352000000bdc19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAEYYACXX_l01n02_smnv0004bronchsup.352000000bdc19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42640348
Sequences flagged as poor quality0
Sequence length101
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC1976010.46341319728441244No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1847420.4332563139494077No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG1081950.2537385482876453No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG1020000.23921005522750424No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG963110.2258682316570212No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC951980.22325802781909757No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC853120.20007341403498863No Hit
CGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCA843930.19791817834132122No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG754920.17704358322779165No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGG700710.16433027235143577No Hit
CGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGC689540.16171068772703262No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC578530.13567666004977258No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC540240.12669690219226168No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG488680.1146050684201733No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG484840.11370451291814036No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG477710.11203238772816769No Hit
CCCGGCCTCTCCCGCCGACCGCGGGCGTGGTGGTGGGGGTGGGGGGGAGG449000.10529932823249942No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC440600.10332936307180232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC190550.086.4099961
TTCGACC220800.075.1731952
TGGCAGG193900.064.91171
GGCAGGT244400.059.6427762
GGGACGC329000.058.6089741
GTTGCGG168500.055.2992441
CCGAGGA194650.053.509322
CTTTCGG177950.052.160751
TTGCGGA170200.049.4694252
CCCCGTG289500.048.8997231
CGGCGAG323050.042.0741581
GCCGAGG449350.039.497451
GCCGGAG168100.038.0323831
TCCGACC95800.034.3852961
GGCGCGA340650.034.1904872
GGGTCGA149600.033.577961
CGGGCTA36450.032.0952341
CTTCGAC115500.031.8966961
TTTCGGT315250.030.3048672
GGCGAGG481350.029.1317422
Produced by FastQC (version 0.11.2)