FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l08n02_perturseq_pilot_run_4.35200000093321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l08n02_perturseq_pilot_run_4.35200000093321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30384168
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC248800.029.36063219
CAGAGTA258700.028.40228518
AAGCAGT268900.027.8882221
GCAGAGT268000.027.4586117
TACATGG269100.027.13722623
GGTATCA278050.026.5836058
CAACGCA279800.026.55033513
CATGGGG185400.025.74909625
GTATCAA288900.025.6451689
AACGCAG293100.025.42235814
AGCAGTG295750.025.2863042
ACATGGG294900.025.09085524
GTGGTAT298450.024.9000686
CGCAGAG298300.024.84274316
TGGTATC300750.024.6093647
GCAGTGG315200.023.7674773
GAGTACA316750.023.41060420
CAGTGGT320250.023.3830344
GGGGATC44800.023.19863328
AGTGGTA329550.022.6646795
Produced by FastQC (version 0.11.2)