FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l08n02_perturseq_pilot_run_3.352000000932df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l08n02_perturseq_pilot_run_3.352000000932df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33146751
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC275650.028.61445619
AAGCAGT291300.027.9483951
CAGAGTA286700.027.75485418
GCAGAGT300200.026.48423617
TACATGG301700.026.24070723
GGTATCA307500.026.0275868
CAACGCA313250.025.73332213
GTATCAA315250.025.4139829
AGCAGTG319550.025.3563392
CGCAGAG325600.024.74963616
CATGGGG210250.024.58904625
ACATGGG326500.024.48848324
AACGCAG331700.024.32235114
GTGGTAT331900.024.2277646
TGGTATC338150.023.8102237
GCAGTGG343900.023.5335253
GAGTACA339350.023.52896720
CAGTGGT353250.022.812264
AGTGGTA363100.022.2016685
GGTCCGG7100.022.17919330
Produced by FastQC (version 0.11.2)