FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l08n02_perturseq_pilot_run_2.35200000093272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l08n02_perturseq_pilot_run_2.35200000093272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46203308
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT415950.028.986781
AGAGTAC408600.028.62554719
CAGAGTA426900.027.5565518
GCAGAGT432600.027.1725717
GGTATCA446100.026.5813778
CAACGCA451000.026.37872713
AACGCAG454250.026.20983514
TACATGG446850.026.1749423
TGGTATC469850.025.2111077
GTATCAA471650.025.1390829
GTGGTAT474400.025.1245236
AGCAGTG484900.024.8172232
CATGGGG306650.024.70151325
CGCAGAG486600.024.44355416
ACATGGG486950.024.35647624
GCAGTGG501650.023.9014723
CAGTGGT509900.023.489794
GGGGATC71600.023.28083428
AGTGGTA522750.022.9212785
GAGTACA519600.022.80090320
Produced by FastQC (version 0.11.2)