FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l08n02_perturseq_pilot_run_1.3520000009324c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l08n02_perturseq_pilot_run_1.3520000009324c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27612057
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC240150.029.16283619
AAGCAGT248000.028.7306231
CAGAGTA249550.028.13645718
GCAGAGT258000.027.35440417
GGTATCA262650.026.9855758
CAACGCA263550.026.9412313
TACATGG262500.026.67953923
AGCAGTG276450.025.8296762
AACGCAG277150.025.64364614
GTATCAA278050.025.4402399
TGGTATC280200.025.3136277
CATGGGG181450.025.26809925
GTGGTAT281100.025.1715036
ACATGGG287050.024.6640624
CGCAGAG290500.024.42562316
GCAGTGG293700.024.2799953
GGGGATC43750.024.01254728
CAGTGGT297350.023.9336414
GAGTACA305100.023.26478620
AGTGGTA310000.022.9967085
Produced by FastQC (version 0.11.2)