FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l08n01_perturseq_pilot_run_4.35100000093324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l08n01_perturseq_pilot_run_4.35100000093324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30384168
Sequences flagged as poor quality0
Sequence length51
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG76500.036.4974868
TGACTAG73850.036.0924341
CTGATCC80400.036.092071
GACCTGG84150.035.981821
GTAACTG74350.035.6680451
CGTGAGC68000.035.456471
GACTAAC64050.035.4287951
TTTACTG55750.035.252051
GCGCGAT78500.035.2449721
CCTTACG69300.035.1811641
GTACTCC91800.035.0432621
CGCTTCA60550.034.8369751
CAACTAG105600.034.727391
CATATTC67650.034.6748731
CCGGGAT101000.034.3921661
TACACGA106650.034.364381
TCGGTAA89800.034.3446581
CCGGTAG83600.034.3066371
ACGAGGA77400.034.204351
ATGCGAT69800.034.090591
Produced by FastQC (version 0.11.2)