FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l08n01_perturseq_pilot_run_3.351000000932d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l08n01_perturseq_pilot_run_3.351000000932d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33146751
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG82100.036.6389128
TGACTAG79600.036.312871
CTGATCC90000.036.0187451
TTTACTG61250.035.8349761
CCTTACG67500.035.785291
GACCTGG89100.035.7509271
GTACTCC93700.035.677591
GACTAAC66500.035.409411
CGCTTCA55150.035.2268561
GTAACTG77800.035.0696641
GCGCGAT79450.035.0213741
ATCGAGT99200.034.9930081
CTCGAAA74800.034.8510251
GGGACCT82100.034.576461
GCTGCAG83250.034.2610741
TACACGA111300.034.2226641
TACGGGC84650.034.093351
CATATTC73850.034.0800251
CAACTAG112500.034.0177041
ACACCGG92200.033.914071
Produced by FastQC (version 0.11.2)