FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l08n01_perturseq_pilot_run_2.35100000093275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l08n01_perturseq_pilot_run_2.35100000093275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46203308
Sequences flagged as poor quality0
Sequence length51
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG120600.036.695078
TGACTAG120100.036.571661
GACCTGG133250.036.3922461
CTGATCC134550.035.956971
GACTAAC105100.035.5792921
CCTTACG102150.035.526881
CATATTC108750.035.5237161
TGCTACC176300.035.3207741
GTAACTG120200.035.155251
TTTACTG91950.035.134241
GCGCGAT117750.035.083711
GTACTCC147700.034.965791
ATCGAGT147500.034.8300441
CAACTAG175200.034.8101041
CGCTTCA87350.034.793751
CGTGAGC109950.034.6445961
CCTACCA111650.034.580861
GACGCCT127850.034.5088410
CCGGGAT160100.034.2262731
ACTGATG119050.034.2089351
Produced by FastQC (version 0.11.2)