FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l08n01_perturseq_pilot_run_1.3510000009324f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l08n01_perturseq_pilot_run_1.3510000009324f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27612057
Sequences flagged as poor quality0
Sequence length51
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCC72750.036.8852771
TGACTAG68600.036.4257621
AGACTAG71250.036.344938
CCTTACG62500.035.802731
CATATTC64000.035.7374461
GACCTGG78300.035.3920821
GTAACTG67650.035.206891
GACTAAC58950.035.132891
TTTACTG55100.035.0546381
TGCTACC106050.034.8768041
CGTGAGC62600.034.8465041
GTACTCC84900.034.7620051
GCGCGAT70300.034.712331
TACACGA99150.034.6928861
CAACTAG99000.034.6772351
GGGACCT70850.034.4110871
TACGGGC72200.034.3912661
CCTACCA62150.034.3381581
CGCTTCA52000.034.157291
CGCTATC48550.034.127021
Produced by FastQC (version 0.11.2)