FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l07n02_input_root_ctrl_rep2_pol-ii.352000000936e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l07n02_input_root_ctrl_rep2_pol-ii.352000000936e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18212069
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT57450.020.9897510
CTTCCGA61750.019.820729
TCCGATC65750.018.71601311
CGTGTGC68500.016.9383891
GTGTGCT71100.016.7307342
TGTGCTC71200.016.7059963
GTGCTCT71600.016.5808854
CCGATCT75800.016.3243812
TCTTCCG82550.014.8840188
GCTCTTC89500.013.4295636
TGCTCTT95100.012.7168885
CTCTTCC121400.010.3249587
CGATCTG64250.08.01872913
AGAGCGT102700.07.140652745
AGCGTCG91400.06.546756745
CGATCTA32700.06.39850413
AAGAGCG126950.06.25507945
GAGCGTC95800.06.05815244
ATCGGAA253550.05.837851545
GAAGAGC166950.05.659181645
Produced by FastQC (version 0.11.2)