FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l07n02_input_root_ctrl_rep1_pol-ii.352000000936a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l07n02_input_root_ctrl_rep1_pol-ii.352000000936a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7342823
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA28800.06.483089445
AGAGCGT61500.06.47432645
GAGCGTC55500.06.36360345
CGTGTAG23750.06.06195645
GCGTCGT50300.05.992847445
GTCGTGT31050.05.86832244
AGCGTCG51450.05.77137244
GAAGAGC92700.05.460082545
AAGAGCG73350.05.458965344
GTAGGGA15653.6379788E-125.318361844
GATCGGA159350.05.308015345
GTGTAGG21750.05.27481645
CGTCGTG45900.05.24405845
TCGGAAG124400.05.17180445
TGTAGGG18050.05.1097945
CTTCCGA15202.4738256E-105.03275359