FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l07n02_chip_root_ctrl_rep3_pol-ii.35200000093562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l07n02_chip_root_ctrl_rep3_pol-ii.35200000093562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10360190
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT21900.012.73891110
CTTCCGA26900.010.4551749
TCCGATC29850.09.27000311
TGTGCTC32500.09.0797833
GTGTGCT33300.08.9976812
CCGATCT33650.08.95904812
GTGCTCT33550.08.3926424
CGTGTGC34850.08.1450371
TCTTCCG41200.07.2648138
TGCTCTT43250.06.8208155
AGAGCGT47950.06.755497544
GAGCGTC49250.06.6229545
GCTCTTC45700.06.59956
GAAGAGC81400.05.85869945
ATCGGAA109800.05.613554545
AAGAGCG60250.05.563126645
CGATCTG28200.05.026128313