FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l07n02_chip_root_ctrl_rep2_pol-ii.35200000093505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l07n02_chip_root_ctrl_rep2_pol-ii.35200000093505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10716138
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT23900.013.46144710
CTTCCGA29250.010.922869
TCCGATC30650.010.78966611
GTGCTCT33550.09.4003674
CCGATCT37250.09.05946212
CGTGTGC36050.08.7494511
GTGTGCT37000.08.5246432
TGTGCTC36500.08.4557323
TCTTCCG42500.07.73091758
AGAGCGT65650.07.23000445
GCTCTTC46600.06.9553786
TGCTCTT46650.06.90354165
GAGCGTC62350.06.34990145
AAGAGCG83750.06.20465945
GTGTAGG25600.05.79955645
ATCGGAA154450.05.44721445
AGCGTCG61350.05.426726345
CGTGTAG30850.05.323024745
TCGTGTA30500.05.2365544
TCGGAAG143050.05.189396445
Produced by FastQC (version 0.11.2)