FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l07n02_chip_root_ctrl_rep1_pol-ii.3520000009349a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l07n02_chip_root_ctrl_rep1_pol-ii.3520000009349a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5260810
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA15350.014.363989
TTCCGAT16200.013.60953910
GTGTGCT16650.012.9888842
TGTGCTC18100.012.0721083
CGTGTGC17550.011.9378141
GTGCTCT18150.011.6662954
TCTTCCG22500.011.0017618
TCCGATC20650.010.24075311
GCTCTTC23750.09.5717746
TGCTCTT24850.08.7903715
CCGATCT26500.08.57480612
GTGTAGG34200.08.28788645
TGTAGGG27550.08.24705945
GTAGGGA22800.08.1892245
CTCTTCC32500.07.409097
CGTGTAG40800.06.891930644
TAGGGAA19800.06.816875545
TCGTGTA44900.06.31252243
GTCGTGT53400.05.644985745
GTGTATC5400.0041962655.42336131