FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l07n01_input_root_ctrl_rep2_pol-ii.351000000936eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l07n01_input_root_ctrl_rep2_pol-ii.351000000936eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18212069
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT63850.06.378380345
ACACGTC47700.06.368074445
AGAGCAC124750.06.330810545
AGCACAC100200.05.90582845
ATCGGAA254850.05.871238745
GCACACG85000.05.77072445
ACGTCTG27550.05.71701345
GAAGAGC170050.05.623478445
CACGTCT43600.05.57352645
GAGCACA109700.05.39423644
AGATCGG343650.05.283843545
AAGAGCA161250.05.26058945
CGGAAGA219650.05.244830645
TCGGAAG225650.05.20508645
GGAAGAG224650.05.128097545
GATCGGA296000.05.09288644