FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l05n02_input_root_ctrl_h2az_05-26-17.352000000936b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l05n02_input_root_ctrl_h2az_05-26-17.352000000936b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30436708
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT179450.06.982840545
AAGAGCG220500.06.580690445
GAGCGTC163250.06.46308745
GTCGTGT95250.06.258946445
TCGTGTA76250.05.900794545
TCGGAAG395200.05.772245
ATCGGAA443400.05.606436745
GATCGGA520150.05.55769745
AGCGTCG153850.05.51258944
GAAGAGC289250.05.506558445
CGGAAGA372950.05.386679645
GCGTCGT158500.05.365162445
AGATCGG599500.05.24612145