FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l05n02_chip_root_ctrl_rep2_h2az_05-26-17.3520000009352f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l05n02_chip_root_ctrl_rep2_h2az_05-26-17.3520000009352f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12041042
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT28750.014.47754910
CTTCCGA33150.013.0337949
GTGCTCT37000.011.6821374
TGTGCTC37900.011.4050083
GTGTGCT38100.011.2865682
TCCGATC37600.011.13032711
CGTGTGC41650.010.26999951
CCGATCT43800.09.86322512
TCTTCCG50650.09.0650978
GCTCTTC50250.08.6457836
TGCTCTT52400.08.24832255
GTGTAGG36900.06.706290745
AGAGCGT107050.06.0523245
GTCGTGT58300.06.019662445
CGTGTAG43100.06.002561645
TGTAGGG27900.05.805563445
CTCTTCC80950.05.75544457
TCGTGTA45850.05.740669745
AAGAGCG127600.05.712291745
GTAGGGA26600.05.666425745