FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l04n02_input_root_ctrl_rep2_h3k4me3.35200000093710.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l04n02_input_root_ctrl_rep2_h3k4me3.35200000093710.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10276517
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT45450.026.77945710
CTTCCGA46250.026.5124959
TCCGATC49150.024.76278111
GTGTGCT50350.024.5904142
GTGCTCT51050.024.4281884
TGTGCTC50650.024.354573
CGTGTGC50650.023.5566651
TCTTCCG58750.020.9876548
CCGATCT60450.020.65496412
GCTCTTC61600.020.0195586
TGCTCTT64000.019.6223555
CTCTTCC76550.016.2843557
CGATCTA23200.011.34555313
CGATCTG51350.07.92986813
CGATCTT40000.07.874007713
CGATCTC56750.06.89780913
GTCGTGT50700.05.94619945
TGTAGGG27800.05.9077345
GTAGGGA23750.05.873144645
CGTGTAG38350.05.690480745
Produced by FastQC (version 0.11.2)