FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l04n02_input_root_ctrl_rep1_h3k4me3.352000000936db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l04n02_input_root_ctrl_rep1_h3k4me3.352000000936db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12269639
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT34400.017.06941210
CTTCCGA37450.015.8002319
TCCGATC40850.014.26362911
GTGCTCT41350.014.10063654
GTGTGCT43050.013.6492372
CGTGTGC41750.013.4279741
TGTGCTC43950.012.9079683
CCGATCT49700.011.95000512
TCTTCCG51600.011.7733048
TGCTCTT52000.011.426575
GCTCTTC54600.011.3760846
GAGTGTC17800.010.8712328
AGTGTCT20050.010.21138829
CTGTGTA20400.09.5941840
CCTGTGT23450.09.40164439
TGTGTAG24550.08.52217141
AGAGTGT24500.08.44932427
CTCTTCC74850.08.2969667
GTGTAGA28250.07.64495842
AAGAGTG27600.07.337255526