FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l04n01_input_root_ctrl_rep1_h3k4me3.351000000936de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l04n01_input_root_ctrl_rep1_h3k4me3.351000000936de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12269639
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT16750.010.74672745
GTCACTA19650.09.50360330
TCTCGTA19900.09.04513344
GTATCTC20500.08.99976141
AGTCACT21050.08.9784229
TATCTCG21900.08.42453742
CAGTCAC22250.08.19082628
TCACTAG22800.07.99324131
TGTATCT26900.07.61132240
GCTTGTA27200.07.361936637
CACTAGC25600.07.03109132
ACTAGCT28250.06.69008833
TTGTATC30750.06.658359539
CCAGTCA28500.06.631429727
ACGTCTG48850.06.54070945
CTTGTAT31300.06.110060738
CACACGT81850.06.10288745
TAGCTTG31800.06.08474435
ATCTCGT33800.06.057605343
CTCCAGT33550.06.03563125
Produced by FastQC (version 0.11.2)