FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l03n02_atac_control_1h_rep2_shoot_06-21-17.352000000932ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l03n02_atac_control_1h_rep2_shoot_06-21-17.352000000932ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46066926
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG35100.014.3652975
TATACAC684850.014.16444445
GTCTAGG81400.013.7747241
CCTAGAC77350.013.330573
CTTATAC1428600.012.75687345
TTATACA1092650.012.71576445
GTACCGT55150.012.2837786
GTCTATG93300.012.2832741
TATACTG132450.012.0154815
ATACACA504050.011.70687345
ACTGTCC55600.011.6154128
AGTACCG74350.011.4441775
CTAGACA114550.011.3400584
TATACCG115550.011.2598245
TCTGACG93550.011.13405745
GTCTTAG73450.011.0967241
TAAGGTG103250.010.9878875
ACCGTGC101700.010.7754538
GACTGTG40250.010.7342067
GTATAAG140750.010.7177691