FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l03n02_atac_control_1h_rep1_shoot_06-07-17.35200000093364.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l03n02_atac_control_1h_rep1_shoot_06-07-17.35200000093364.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48740450
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG37350.015.006555
CCTAGAC81750.014.6508673
GTCTAGG90850.014.4980211
TATACAC643150.013.96005445
GTCTATG102450.013.8454281
CTTATAC1387550.013.72772345
GTACCGT59100.012.7957316
ACTGTCC61950.012.6403848
CTAGACA112450.012.3325694
ACTGAGC127100.012.2690258
TTATACA1046950.012.22225345
GGTCCGT63850.012.19255511
TATACCG130800.012.1497995
TATACTG132350.012.1265625
AGTACCG85650.011.9842155
GTATAAG142450.011.9807641
ATACACA472050.011.61408945
CACTGAG142250.011.61107357
GTATAGG128900.011.5982641
GGACCGT65550.011.4336486