FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l03n02_atac_control_1h_rep1_root_06-15-17.352000000933a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l03n02_atac_control_1h_rep1_root_06-15-17.352000000933a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32597282
Sequences flagged as poor quality0
Sequence length51
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC559100.016.71460745
ATACACA353550.014.41240845
TTATACA810600.013.92376645
TATACTG14050.012.0156915
TACACAT241550.011.39038945
CTTATAC1077700.011.1784345
TAGACAG13500.011.1713285
GTCTAGG14800.010.95323851
TCTGACG106450.010.79927145
TACACCG96050.010.4754795
CTAGACT10500.010.2914934
CTATACT16100.010.2075954
GTTCTAG11600.010.0929261
TAGGACT15000.09.6053934
ATCTGAC119000.09.60355444
GTCCTAG10850.09.5455261
GTATATA16350.09.50173951
GTCTAAG9500.09.4799971
TCTTATA1363750.09.47542845
CCTACAC60950.09.2711823