FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l03n01_atac_control_1h_rep2_shoot_06-21-17.351000000932ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l03n01_atac_control_1h_rep2_shoot_06-21-17.351000000932ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46066926
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTC67100.016.08014140
TATACAC662300.014.34058145
GTGTCTC91400.013.541759541
CCTAGAC82650.013.394223
GTCTAGG82000.013.2040781
CAGTCTC76850.012.96234540
GTACCGT58500.012.947696
CTTATAC1384400.012.79008845
TATACTG128300.012.750685
TTATACA1064450.012.68804645
TAGACGG39400.012.6268175
AGTACCG78300.012.5357265
GTCTATG99450.012.1269691
GTATAGG133700.011.9880741
ATACACA489550.011.69463945
ACTGTCC58450.011.6548418
CTAGACA115050.011.5310764
ACTGAGC108050.011.531018
GTCTTAG77400.011.4980541
TATACCG115700.011.4857255