FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l03n01_atac_control_1h_rep1_shoot_06-07-17.35100000093367.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l03n01_atac_control_1h_rep1_shoot_06-07-17.35100000093367.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48740450
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTC59450.016.91001940
TAGACGG38600.014.6303555
GTCTAGG94750.014.1956091
TATACAC620200.014.175014545
GTACCGT60350.013.9248566
CTTATAC1343750.013.7453745
CCTAGAC83800.013.5045913
GTGTCTC96150.013.11131441
TATACTG135200.013.0448675
GTCTATG108950.012.942121
AGTACCG86200.012.76485355
CTAGACA115900.012.6452774
ACTGTCC61300.012.3561128
TTATACA1010950.012.25705245
CAGTCTC79800.012.13650740
ACTGAGC129450.011.9273178
ATACACA448900.011.8993245
GTATAAG144050.011.8738981
TAAGGTG116200.011.8607085
GACAGTG58500.011.8366337