FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l03n01_atac_control_1h_rep1_root_06-15-17.351000000933ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l03n01_atac_control_1h_rep1_root_06-15-17.351000000933ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32597282
Sequences flagged as poor quality0
Sequence length51
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTTA19200.024.67190641
TGTCTTA21000.023.6521840
GCTCTTA25850.020.10409741
GTCTTAT26300.019.14807341
AGTCTCT28300.018.7699141
TATCTTA23100.018.71468740
GACTCTT27200.018.17633240
TATACAC532550.017.05280745
GCTTATA19950.016.021741
GTATCTT27400.015.94550839
ATCTTAT27700.015.93889241
TGACTCT31200.015.55117539
GGTCTCT45150.015.12639441
ATACACA336950.014.70350545
TATCTCT41350.014.57009840
TCGCTTA22300.014.23015239
TTATACA776050.014.03336545
TGTGTCT37450.013.876883538
ATGTCTC41050.013.55622941
TGTATCT34050.013.37160138
Produced by FastQC (version 0.11.2)