FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l02n02_atac_control_1h_rep1_root_06-21-17.35200000093259.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l02n02_atac_control_1h_rep1_root_06-21-17.35200000093259.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9861973
Sequences flagged as poor quality0
Sequence length51
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC228350.015.50635345
TCTAGAC1901.3203862E-714.2235973
ATACACA150850.013.60051845
TATAAGA2703.2923708E-1013.33341513
TTATACA323650.013.317595545
TAAGAGA2959.640644E-1112.96618215
CTAGAAC2104.4678563E-712.868973
ATAAGAG3202.7284841E-1112.65632814
GTATAAG2951.3369572E-912.20321812
CTACACT3001.718945E-912.0108564
TGTATAA3052.2646418E-911.80203511
TAGGACC2502.884408E-711.7105844
GTGTATA3102.924935E-911.61150410
TCTGACG39300.011.44836245
CTAGACT2402.2419572E-611.2601774
GTGTAAA2707.780109E-710.8454451
CCTAGAA2301.7182982E-510.7716432
TTAGACC2108.1115766E-510.7241413
TTCTAAG1903.8368782E-410.6685652
TAGACTG2759.912492E-710.6433375