FastQCFastQC Report
Mon 3 Jul 2017
CA4RDACXX_l01n02_atac_control_1h_rep2_shoot_06-13-17.3520000009323f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCA4RDACXX_l01n02_atac_control_1h_rep2_shoot_06-13-17.3520000009323f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92435876
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2831050.015.09696945
TAGACGG83050.014.73121555
TATACAC1369850.014.34168545
TTATACA2065200.013.73285145
TCTTATA4082350.013.086596545
GTCTAGG172600.013.0282761
CCTAGAC160700.012.6573343
TATACCG245400.012.5181895
CTAGACA248300.012.3634084
TATACTG228200.012.1703315
TCTGACG177450.012.04147145
ATACACA1014350.012.02404545
GTCTATG203750.011.858451
ACTGTCC149700.011.7026718
TAGAGCG190350.011.5291765
GTATAGG245400.011.4928321
GTACCGT145650.011.4631846
CTCTTAT5364900.011.1833145
CCTATAC257700.011.1767373
TAAGGTG239650.011.1696455