v1.0.dev0

Loading report..

Toolbox

MultiQC Toolbox

Highlight Samples

This report has flat image plots that won't be highlighted.
See the documentation for help.

Regex mode off

Rename Samples

This report has flat image plots that won't be renamed.
See the documentation for help.

Click here for bulk input.

Paste two columns of a tab-delimited table here (eg. from Excel).

First column should be the old name, second column the new name.

Regex mode off

Show / Hide Samples

This report has flat image plots that won't be hidden.
See the documentation for help.

Regex mode off

Export Plots

Images
Data

px

px

Aspect ratio

Plot scaling

X

Download the raw data used to create the plots in this report below:

Format:

Note that additional data was saved in multiqc_data when this report was generated.

Choose Plots

If you use plots from MultiQC in a publication or presentation, please cite:

MultiQC: Summarize analysis results for multiple tools and samples in a single report
Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
Bioinformatics (2016)
doi: 10.1093/bioinformatics/btw354
PMID: 27312411

Save Settings

You can save the toolbox settings for this report to the browser.

Load Settings

Choose a saved report profile from the dropdown box below:

About MultiQC

This report was generated using MultiQC, version 1.0.dev0

You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

MultiQC is published in Bioinformatics:

MultiQC: Summarize analysis results for multiple tools and samples in a single report
Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
Bioinformatics (2016)
doi: 10.1093/bioinformatics/btw354
PMID: 27312411

A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

Loading report..

Report generated on 2017-05-16, 16:05 based on data in: /mnt/gencore/sites/core-fastqc.bio.nyu.edu/html/C9LUHACXX

General Statistics

Showing ¹²⁰/₁₂₀ rows and ³/₅ columns.

Sample Name	% Dups	% GC	Length	% Failed	M Seqs
C9LUHACXX_l01n01_osat_119.3510000008cd55	28.2%	45%	101 bp	25%	32.7
C9LUHACXX_l01n01_osat_140.3510000008cddb	24.9%	42%	101 bp	33%	18.8
C9LUHACXX_l01n01_osat_144.3510000008ce54	33.2%	45%	101 bp	33%	58.3
C9LUHACXX_l01n01_osat_150.3510000008ceda	27.6%	43%	101 bp	25%	9.0
C9LUHACXX_l01n01_osat_183.3510000008cf53	25.6%	45%	101 bp	33%	30.0
C9LUHACXX_l01n01_osat_204.3510000008cfd9	29.3%	46%	101 bp	33%	35.0
C9LUHACXX_l01n01_osat_84.3510000008cd11	24.4%	45%	101 bp	33%	53.9
C9LUHACXX_l01n02_osat_119.3520000008cd52	27.1%	45%	101 bp	33%	32.7
C9LUHACXX_l01n02_osat_140.3520000008cdd8	23.8%	42%	101 bp	33%	18.8
C9LUHACXX_l01n02_osat_144.3520000008ce51	31.9%	45%	101 bp	33%	58.3
C9LUHACXX_l01n02_osat_150.3520000008ced7	26.5%	43%	101 bp	25%	9.0
C9LUHACXX_l01n02_osat_183.3520000008cf50	24.5%	45%	101 bp	33%	30.0
C9LUHACXX_l01n02_osat_204.3520000008cfd6	28.4%	46%	101 bp	33%	35.0
C9LUHACXX_l01n02_osat_84.3520000008cd1e	23.5%	45%	101 bp	33%	53.9
C9LUHACXX_l02n01_osat_104.3510000008cdce	28.0%	42%	101 bp	17%	24.9
C9LUHACXX_l02n01_osat_111.3510000008cfcc	27.5%	44%	101 bp	25%	27.6
C9LUHACXX_l02n01_osat_137.3510000008d25d	21.9%	41%	101 bp	17%	12.1
C9LUHACXX_l02n01_osat_164.3510000008cecd	27.5%	43%	101 bp	17%	27.2
C9LUHACXX_l02n01_osat_182.3510000008d042	35.1%	44%	101 bp	17%	62.1
C9LUHACXX_l02n01_osat_4.3510000008d2d3	16.9%	42%	101 bp	17%	10.3
C9LUHACXX_l02n01_osat_41.3510000008d141	26.2%	41%	101 bp	17%	17.5
C9LUHACXX_l02n01_osat_59.3510000008cf46	29.0%	41%	101 bp	17%	19.7
C9LUHACXX_l02n01_osat_61.3510000008ce47	22.0%	42%	101 bp	25%	9.3
C9LUHACXX_l02n01_osat_62.3510000008d0c8	26.0%	40%	101 bp	17%	21.7
C9LUHACXX_l02n01_osat_76.3510000008d1d4	24.8%	40%	101 bp	17%	16.6
C9LUHACXX_l02n02_osat_104.3520000008cdcb	26.7%	43%	101 bp	17%	24.9
C9LUHACXX_l02n02_osat_111.3520000008cfc9	26.3%	44%	101 bp	25%	27.6
C9LUHACXX_l02n02_osat_137.3520000008d25a	20.8%	41%	101 bp	17%	12.1
C9LUHACXX_l02n02_osat_164.3520000008ceca	25.5%	43%	101 bp	17%	27.2
C9LUHACXX_l02n02_osat_182.3520000008d04f	33.1%	44%	101 bp	17%	62.1
C9LUHACXX_l02n02_osat_4.3520000008d2d0	16.4%	42%	101 bp	17%	10.3
C9LUHACXX_l02n02_osat_41.3520000008d14e	25.0%	41%	101 bp	17%	17.5
C9LUHACXX_l02n02_osat_59.3520000008cf43	27.7%	41%	101 bp	17%	19.7
C9LUHACXX_l02n02_osat_61.3520000008ce44	21.2%	42%	101 bp	25%	9.3
C9LUHACXX_l02n02_osat_62.3520000008d0c5	25.5%	40%	101 bp	17%	21.7
C9LUHACXX_l02n02_osat_76.3520000008d1d1	24.2%	40%	101 bp	17%	16.6
C9LUHACXX_l03n01_ogla_115.3510000008d085	19.8%	44%	101 bp	25%	12.6
C9LUHACXX_l03n01_ogla_116.3510000008d01b	27.0%	44%	101 bp	25%	29.4
C9LUHACXX_l03n01_ogla_117.3510000008cfa3	24.0%	44%	101 bp	25%	25.3
C9LUHACXX_l03n01_ogla_118.3510000008cf2c	24.2%	44%	101 bp	25%	24.5
C9LUHACXX_l03n01_ogla_119.3510000008cea4	31.2%	43%	101 bp	25%	45.5
C9LUHACXX_l03n01_ogla_121.3510000008ce2d	31.4%	44%	101 bp	25%	51.2
C9LUHACXX_l03n01_ogla_122.3510000008cda5	36.7%	44%	101 bp	25%	78.7
C9LUHACXX_l03n02_ogla_115.3520000008d082	18.5%	44%	101 bp	25%	12.6
C9LUHACXX_l03n02_ogla_116.3520000008d018	25.8%	44%	101 bp	25%	29.4
C9LUHACXX_l03n02_ogla_117.3520000008cfa0	22.5%	44%	101 bp	33%	25.3
C9LUHACXX_l03n02_ogla_118.3520000008cf29	23.0%	44%	101 bp	25%	24.5
C9LUHACXX_l03n02_ogla_119.3520000008cea1	30.9%	43%	101 bp	25%	45.5
C9LUHACXX_l03n02_ogla_121.3520000008ce2a	30.4%	44%	101 bp	25%	51.2
C9LUHACXX_l03n02_ogla_122.3520000008cda2	36.1%	44%	101 bp	25%	78.7
C9LUHACXX_l04n01_ogla_106.3510000008cf7c	25.0%	44%	101 bp	33%	29.6
C9LUHACXX_l04n01_ogla_107.3510000008cef4	25.7%	44%	101 bp	25%	28.6
C9LUHACXX_l04n01_ogla_111.3510000008ce7d	28.0%	43%	101 bp	33%	30.8
C9LUHACXX_l04n01_ogla_112.3510000008cdf5	25.1%	45%	101 bp	25%	23.8
C9LUHACXX_l04n01_ogla_113.3510000008cd8b	28.5%	45%	101 bp	25%	29.7
C9LUHACXX_l04n01_ogla_114.3510000008cd3b	24.8%	44%	101 bp	33%	26.3
C9LUHACXX_l04n01_ogla_tva1.3510000008cfe6	21.1%	44%	101 bp	33%	25.6
C9LUHACXX_l04n02_ogla_106.3520000008cf79	25.0%	44%	101 bp	25%	29.6
C9LUHACXX_l04n02_ogla_107.3520000008cef1	25.6%	44%	101 bp	25%	28.6
C9LUHACXX_l04n02_ogla_111.3520000008ce7a	28.1%	44%	101 bp	25%	30.8
C9LUHACXX_l04n02_ogla_112.3520000008cdf2	25.3%	45%	101 bp	17%	23.8
C9LUHACXX_l04n02_ogla_113.3520000008cd88	28.1%	45%	101 bp	17%	29.7
C9LUHACXX_l04n02_ogla_114.3520000008cd38	24.3%	44%	101 bp	25%	26.3
C9LUHACXX_l04n02_ogla_tva1.3520000008cfe3	21.3%	44%	101 bp	25%	25.6
C9LUHACXX_l05n01_ogla_100.3510000008cf89	23.4%	45%	101 bp	25%	20.3
C9LUHACXX_l05n01_ogla_101.3510000008cf02	25.5%	45%	101 bp	25%	28.2
C9LUHACXX_l05n01_ogla_102.3510000008ce8a	19.1%	45%	101 bp	25%	12.6
C9LUHACXX_l05n01_ogla_103.3510000008ce03	22.6%	45%	101 bp	25%	16.3
C9LUHACXX_l05n01_ogla_105.3510000008cd7e	21.2%	44%	101 bp	25%	16.4
C9LUHACXX_l05n01_ogla_98.3510000008d06b	23.6%	45%	101 bp	25%	24.4
C9LUHACXX_l05n01_ogla_99.3510000008cff3	21.7%	45%	101 bp	25%	17.7
C9LUHACXX_l05n02_ogla_100.3520000008cf86	22.2%	45%	101 bp	33%	20.3
C9LUHACXX_l05n02_ogla_101.3520000008cf0f	25.0%	45%	101 bp	25%	28.2
C9LUHACXX_l05n02_ogla_102.3520000008ce87	19.0%	46%	101 bp	33%	12.6
C9LUHACXX_l05n02_ogla_103.3520000008ce00	22.2%	45%	101 bp	25%	16.3
C9LUHACXX_l05n02_ogla_105.3520000008cd7b	20.6%	44%	101 bp	25%	16.4
C9LUHACXX_l05n02_ogla_98.3520000008d068	23.4%	45%	101 bp	33%	24.4
C9LUHACXX_l05n02_ogla_99.3520000008cff0	21.2%	46%	101 bp	25%	17.7
C9LUHACXX_l06n01_ogla_90.3510000008cf39	23.3%	44%	101 bp	25%	25.4
C9LUHACXX_l06n01_ogla_92.3510000008ceb0	26.7%	45%	101 bp	25%	18.5
C9LUHACXX_l06n01_ogla_93.3510000008ce3a	23.9%	46%	101 bp	25%	18.9
C9LUHACXX_l06n01_ogla_94.3510000008cdb1	21.3%	44%	101 bp	25%	20.7
C9LUHACXX_l06n01_ogla_95.3510000008cd48	22.6%	44%	101 bp	17%	20.5
C9LUHACXX_l06n01_ogla_96.3510000008cd2e	24.8%	44%	101 bp	25%	26.2
C9LUHACXX_l06n01_ogla_97.3510000008ccf6	25.9%	45%	101 bp	25%	22.1
C9LUHACXX_l06n02_ogla_90.3520000008cf36	23.3%	44%	101 bp	25%	25.4
C9LUHACXX_l06n02_ogla_92.3520000008cebd	25.9%	45%	101 bp	25%	18.5
C9LUHACXX_l06n02_ogla_93.3520000008ce37	24.0%	46%	101 bp	25%	18.9
C9LUHACXX_l06n02_ogla_94.3520000008cdbe	21.7%	44%	101 bp	25%	20.7
C9LUHACXX_l06n02_ogla_95.3520000008cd45	22.4%	44%	101 bp	25%	20.5
C9LUHACXX_l06n02_ogla_96.3520000008cd2b	25.2%	44%	101 bp	25%	26.2
C9LUHACXX_l06n02_ogla_97.3520000008ccf3	26.0%	45%	101 bp	25%	22.1
C9LUHACXX_l07n01_ogla_78.3510000008d092	22.3%	44%	101 bp	25%	20.4
C9LUHACXX_l07n01_ogla_80.3510000008d00e	22.7%	44%	101 bp	25%	19.7
C9LUHACXX_l07n01_ogla_81.3510000008cf6f	25.3%	45%	101 bp	25%	21.2
C9LUHACXX_l07n01_ogla_83.3510000008cee7	22.6%	44%	101 bp	25%	15.6
C9LUHACXX_l07n01_ogla_84.3510000008ce60	21.1%	44%	101 bp	25%	14.0
C9LUHACXX_l07n01_ogla_85.3510000008cde8	26.2%	44%	101 bp	25%	19.7
C9LUHACXX_l07n01_ogla_88.3510000008cd61	23.5%	45%	101 bp	25%	18.2
C9LUHACXX_l07n02_ogla_78.3520000008d09f	21.9%	45%	101 bp	25%	20.4
C9LUHACXX_l07n02_ogla_80.3520000008d00b	22.7%	44%	101 bp	25%	19.7
C9LUHACXX_l07n02_ogla_81.3520000008cf6c	25.4%	45%	101 bp	25%	21.2
C9LUHACXX_l07n02_ogla_83.3520000008cee4	22.6%	45%	101 bp	25%	15.6
C9LUHACXX_l07n02_ogla_84.3520000008ce6d	21.8%	44%	101 bp	25%	14.0
C9LUHACXX_l07n02_ogla_85.3520000008cde5	25.3%	45%	101 bp	25%	19.7
C9LUHACXX_l07n02_ogla_88.3520000008cd6e	23.3%	45%	101 bp	25%	18.2
C9LUHACXX_l08n01_ogla_69.3510000008d0af	26.0%	44%	101 bp	33%	36.4
C9LUHACXX_l08n01_ogla_71.3510000008d028	26.2%	45%	101 bp	25%	28.4
C9LUHACXX_l08n01_ogla_73.3510000008cf96	26.9%	46%	101 bp	25%	23.9
C9LUHACXX_l08n01_ogla_74.3510000008cf1f	27.1%	46%	101 bp	25%	24.6
C9LUHACXX_l08n01_ogla_75.3510000008ce97	25.0%	44%	101 bp	17%	17.4
C9LUHACXX_l08n01_ogla_76.3510000008ce10	24.7%	44%	101 bp	25%	25.0
C9LUHACXX_l08n01_ogla_77.3510000008cd98	22.3%	44%	101 bp	25%	20.7
C9LUHACXX_l08n02_ogla_69.3520000008d0ac	26.3%	44%	101 bp	33%	36.4
C9LUHACXX_l08n02_ogla_71.3520000008d025	25.5%	45%	101 bp	25%	28.4
C9LUHACXX_l08n02_ogla_73.3520000008cf93	26.5%	46%	101 bp	25%	23.9
C9LUHACXX_l08n02_ogla_74.3520000008cf1c	26.7%	46%	101 bp	25%	24.6
C9LUHACXX_l08n02_ogla_75.3520000008ce94	24.4%	44%	101 bp	25%	17.4
C9LUHACXX_l08n02_ogla_76.3520000008ce1d	24.4%	44%	101 bp	25%	25.0
C9LUHACXX_l08n02_ogla_77.3520000008cd95	21.7%	45%	101 bp	25%	20.7

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|		FastQC	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|		FastQC	% GC	Average % GC Content	`percent_gc`	None
\|\|		FastQC	Length	Average Sequence Length (bp)	`avg_sequence_length`	None
\|\|		FastQC	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|		FastQC	M Seqs	Total Sequences (millions)	`total_sequences`	read_count

Lane 1 Demultiplexing Report

Lane 1 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.
Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

Showing ⁷/₇ rows and ³/₃ columns.

Library	Total Read Count	Portion (%)	Perfect (%)
osat_140	18756721	7.9	98.3
osat_183	29958202	12.6	99.2
osat_119	32650088	13.7	98.9
osat_204	34978160	14.7	99.0
osat_150	9006792	3.8	98.7
osat_144	58344761	24.6	99.3
osat_84	53924047	22.7	99.0

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None
\|\|			Perfect (%)	Perfect (%)	`Perfect (%)`	None

Lane 3 Demultiplexing Report

Lane 3 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.
Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

Showing ⁷/₇ rows and ³/₃ columns.

Library	Total Read Count	Portion (%)	Perfect (%)
ogla_115	12642461	4.7	99.1
ogla_117	25331709	9.5	98.8
ogla_119	45529225	17.0	99.2
ogla_121	51155991	19.1	99.1
ogla_118	24471567	9.2	99.2
ogla_122	78740934	29.5	99.4
ogla_116	29367821	11.0	99.2

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None
\|\|			Perfect (%)	Perfect (%)	`Perfect (%)`	None

Lane 2 Demultiplexing Report

Lane 2 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.
Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

Showing ¹¹/₁₁ rows and ³/₃ columns.

Library	Total Read Count	Portion (%)	Perfect (%)
osat_182	62101033	25.0	99.2
osat_41	17469344	7.0	98.7
osat_59	19652835	7.9	99.2
osat_137	12079105	4.9	99.3
osat_61	9291896	3.7	98.9
osat_164	27192019	10.9	99.4
osat_111	27628206	11.1	99.2
osat_4	10260648	4.1	98.8
osat_76	16558542	6.7	99.3
osat_62	21675973	8.7	99.2
osat_104	24888989	10.0	99.4

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None
\|\|			Perfect (%)	Perfect (%)	`Perfect (%)`	None

Lane 7 Demultiplexing Report

Lane 7 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.
Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

Showing ⁷/₇ rows and ³/₃ columns.

Library	Total Read Count	Portion (%)	Perfect (%)
ogla_88	18182813	14.1	99.5
ogla_81	21219435	16.5	99.2
ogla_83	15638679	12.1	99.4
ogla_85	19696905	15.3	99.1
ogla_84	14027871	10.9	93.9
ogla_78	20387775	15.8	99.2
ogla_80	19663821	15.3	99.3

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None
\|\|			Perfect (%)	Perfect (%)	`Perfect (%)`	None

Lane 6 Demultiplexing Report

Lane 6 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.
Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

Showing ⁷/₇ rows and ³/₃ columns.

Library	Total Read Count	Portion (%)	Perfect (%)
ogla_94	20692204	13.6	98.8
ogla_93	18864497	12.4	98.8
ogla_95	20531097	13.5	98.7
ogla_96	26240646	17.2	98.5
ogla_92	18471973	12.1	98.8
ogla_90	25390306	16.7	98.6
ogla_97	22126982	14.5	99.0

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None
\|\|			Perfect (%)	Perfect (%)	`Perfect (%)`	None

Lane 5 Demultiplexing Report

Lane 5 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.
Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

Showing ⁷/₇ rows and ³/₃ columns.

Library	Total Read Count	Portion (%)	Perfect (%)
ogla_98	24365529	17.9	99.2
ogla_102	12610236	9.3	91.4
ogla_101	28233776	20.8	99.4
ogla_100	20289905	14.9	99.0
ogla_105	16429835	12.1	99.3
ogla_99	17685336	13.0	99.3
ogla_103	16297591	12.0	94.9

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None
\|\|			Perfect (%)	Perfect (%)	`Perfect (%)`	None

Lane 8 Demultiplexing Report

Lane 8 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.
Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

Showing ⁷/₇ rows and ³/₃ columns.

Library	Total Read Count	Portion (%)	Perfect (%)
ogla_69	36411131	20.7	99.2
ogla_73	23872333	13.5	99.3
ogla_75	17362524	9.8	93.1
ogla_77	20710531	11.7	99.5
ogla_71	28416542	16.1	99.3
ogla_76	24979078	14.2	99.1
ogla_74	24566394	13.9	99.4

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None
\|\|			Perfect (%)	Perfect (%)	`Perfect (%)`	None

Lane 4 Demultiplexing Report

Lane 4 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.
Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

Showing ⁷/₇ rows and ³/₃ columns.

Library	Total Read Count	Portion (%)	Perfect (%)
ogla_111	30846502	15.9	99.3
ogla_tva1	25587062	13.2	99.0
ogla_112	23830559	12.3	98.0
ogla_106	29550054	15.2	99.1
ogla_114	26261131	13.5	99.3
ogla_107	28594662	14.7	99.0
ogla_113	29665809	15.3	99.0

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None
\|\|			Perfect (%)	Perfect (%)	`Perfect (%)`	None

Lane Statistics

Lane Statistics

Showing ⁸/₈ rows and ³/₃ columns.

Lane	Total # of Single-End Reads	Total # PF Reads	Undetermined
1.0	254232238	241941049	1.79%
2.0	269830901	253730079	1.94%
3.0	290790755	272032154	1.76%
4.0	207605805	199056385	2.37%
5.0	146366998	139778763	2.77%
6.0	160423363	156273147	2.53%
7.0	136548092	133379897	3.42%
8.0	187144618	181498059	2.85%

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total # of Single-End Reads	Total # of Single-End Reads	`Total # of Single-End Reads`	None
\|\|			Total # PF Reads	Total # PF Reads	`Total # PF Reads`	None
\|\|			Undetermined	Undetermined	`Undetermined`	None

FastQC

FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

Sequence Quality Histograms

The mean quality value across each base position in the read. See the FastQC help.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

Click a heatmap row to see a line plot for that dataset.

rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Base N Content

The percentage of base calls at each position for which an N was called. See the FastQC help.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Sequence Length Distribution

All samples have sequences of a single length (101bp).

Sequence Duplication Levels

The relative level of duplication found for every sequence. See the FastQC help.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Overrepresented sequences

The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).