FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l08n02_ogla_77.3520000008cd95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l08n02_ogla_77.3520000008cd95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20710531
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG125950.015.6122055
GTATAGG85300.014.9229311
TAGACTG67700.014.522585
GTATTGG198650.014.1547281
TATACCG67850.013.6504465
TATACAG118850.013.587575
GTATATA272450.013.5806021
CTATACT126950.013.4688924
GTATAAG92700.013.2705341
GTATTAG100850.012.9516521
GTATAAT146450.012.7783641
ATACTGT130900.012.6996136
TTATACT158100.012.3473014
GTATTAT147850.012.3039861
TAATACT164900.012.2125774
TACACTG91350.012.1146115
TCTATAC105800.012.0762423
TATTGGG217000.011.9071422
TACACCG70600.011.7732255
GTATTAC88700.011.6735051
Produced by FastQC (version 0.11.2)