FastQCFastQC Report
Mon 15 May 2017
C9LUHACXX_l08n02_ogla_76.3520000008ce1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUHACXX_l08n02_ogla_76.3520000008ce1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24979078
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG153600.017.1925015
GTATATA341000.015.73918151
GTATAGG113350.015.421
GTATAAG113250.015.0561641
TATACCG73700.015.0156425
TATACAG137950.014.0129055
CCTATAC109500.013.7500163
GTATAGA131050.013.6997571
CTATACT159250.013.5105884
TTATACT191500.013.3930814
CCTAGAC83950.013.3520953
GTATTGG192300.013.3127451
TACACTG113300.013.2887725
GTATAAT181850.013.2680921
ATACTGT157800.013.2434516
GTATTAG126950.012.7205031
ATTATAC174150.012.6001753
TAGACTG77750.012.5841055
TCTATAC128250.012.5174953
GTATTAA170900.012.47851